Genome Editing Toolbox

Genome Editing



Breakthrough Technologies A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation

Department of Biology, East Carolina University, Greenville, North Carolina 27858 (L.G.L., J.W.P., Y.Q.); Department of Biotechnology, School of Life Sciences and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China (D.Z., X.T., X.Z., Y.Z.); Department of Genetics, Cell Biology, and Development and Center for Genome Engineering, University of Minnesota, Mi...

Development of a CRISPR/Cas9 genome editing toolbox for Corynebacterium glutamicum

BACKGROUND Corynebacterium glutamicum is an important industrial workhorse and advanced genetic engineering tools are urgently demanded. Recently, the clustered regularly interspaced short palindromic repeats (CRISPR) and their CRISPR-associated proteins (Cas) have revolutionized the field of genome engineering. The CRISPR/Cas9 system that utilizes NGG as protospacer adjacent motif (PAM) and ha...

A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation.

The relative ease, speed, and biological scope of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated Protein9 (Cas9)-based reagents for genomic manipulations are revolutionizing virtually all areas of molecular biosciences, including functional genomics, genetics, applied biomedical research, and agricultural biotechnology. In plant systems, however, a number o...

A Comprehensive Toolbox for Genome Editing in Cultured Drosophila melanogaster Cells

Custom genome editing has become an essential element of molecular biology. In particular, the generation of fusion constructs with epitope tags or fluorescent proteins at the genomic locus facilitates the analysis of protein expression, localization, and interaction partners at physiologic levels. Following up on our initial publication, we now describe a considerably simplified, more efficien...